The North American freshwater mussel tribe Quadrulini has a suite of life history adaptations, however, the evolution of life histories in this group has yet to be explored using comparative phylogenetic methods. Here we provide the AHE DNA sequence alignment representing 27 species from the subfamily Ambleminae that was used to estimate a phylogenomic reconstruction of Quadrulini. Additionally, we provide a compilation of host use information and larval length and height measurements utilized to trace the evolution of life history characteristics, such as larval morphology and host attraction strategy. Phylogenetic trees are presented representing phylogenetic inference performed under Maximum Likelihood, Maximum Parsimony, Bayesian Inference, and coalescent-based approaches. Findings from these datasets and analyses suggest the diversification of Quadrulini has been influenced by the codiversification of their larval hosts.