The U.S. Geological Survey Precipitation-Runoff Modeling System (PRMS) was used to assess the effects of changing climate and land disturbance on seasonal streamflow in the Rio Grande Headwaters (RGHW) region. Three applications of PRMS in the RGHW were used to simulate 1) baseline effects of climate (see RGHW-PRMS_baseline_input.zip), 2) effects of bark-beetle induced tree mortality (see RGHW-PRMS_BB_input.zip), and 3) effects of wildfire (see RGHW-PRMS_fire_input.zip), on components of the hydrologic cycle by hydrologic response unit (HRU) and subsequent seasonal streamflow runoff from April through September for water years 1980 through 2017. PRMS input files (control, climate-by-hru, data, parameter, dynamic parameter, and initial condition, where applicable) for each simulation are contained in this data release. Common input used in each simulation (climate_by_hru, static parameter, and data files) are contained in the RGHW-PRMS_common_input.zip file.