The North of Interstate 40 Pronghorn herd primarily resides in Arizona’s Game Management Unit (GMU) 7. GMU 7 had an estimated population of 550 pronghorn in 2019. The Pronghorn North of Interstate 40 summer in high elevation open meadows and ponderosa pine habitat near Government Prairie. When winter conditions set in the pronghorn (Antilocapra americana) seek lower elevations, migrating through mixed pinyon-juniper woodlands to open grassland and shrub habitats north of Sitgreaves Mountain, often crossing US Highway 180 (US-180) towards Antelope Flat. US-180 is an increasing threat to this migration corridor as traffic volumes rise. However, right-of-way fence improvements and relatively low traffic volumes on US-180 currently allow some degree of permeability across this highway. Additionally, encroaching pinyon-juniper woodlands are a threat to open grassland habitat within both summer and winter range. These data provide the location of migration routes for American pronghorn from the North of Interstate 40 Herd in Arizona. They were developed from 65 migration sequences collected from a sample size of 16 animals comprising GPS locations collected every 2 hours.