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Microbial Populations in PFHxSAm (perfluorohexane sulfonamido propyl amine) Biodegradation Microcosms

Metadata Updated: October 29, 2023

Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but the microbial populations catalyzing degradation are poorly characterized. We conducted laboratory microcosm experiments to investigate the biotransformation of perfluorohexane sulfonamido propyl amine (PFHxSAm), a predominant precursor in 3M Aqueous Film Forming Foam (3M AFFF), by native microbial populations in upwelling sediment-water slurries from Ashumet Pond, MA. Ashumet Pond is affected by multiple PFAS-contaminated groundwater plumes from Joint Base Cape Cod, MA. The field collected samples were used to construct aerobic microcosms for monitoring PFHxSAm biodegradation. Microbial community composition was analyzed at 4 timepoints using Illumina 16S rRNA gene iTag sequencing and quantitative PCR (qPCR) targeting bacterial 16S rRNA genes. Experiments were performed in triplicate and abiotic controls were included in each experiment. The data release contains the results of Illumina iTag sequencing and qPCR for microbial community characterization. Four tables are provided that describe the experimental bottles, the results of DNA quantification, the results of biomass estimates using qPCR, and the taxonomic data based on sequencing results (provided as a biom file).

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Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023
Publisher U.S. Geological Survey
Identifier USGS:62a36f05d34ec53d2770bd60
Data Last Modified 20221118
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id 62c1e0b8-2182-4996-89c5-8bafed748b32
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -70.6448,41.6154,-70.4141,41.7877
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 57292ee948c33b87fa12350985cdc73d035bd0eb31884de3cc3bfb607bb151c8
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -70.6448, 41.6154, -70.6448, 41.7877, -70.4141, 41.7877, -70.4141, 41.6154, -70.6448, 41.6154}

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