The Colorado Plateau is associated with several different commercial sources of natural CO2 and other non-hydrocarbon gases, such as noble gases. Twenty-five different hot springs, warm springs, cold springs, and/or rivers across the Utah region of the Colorado Plateau were sampled for microbial biomass. These different locations were associated with some concentration of dissolved or effervescing natural CO2 or other naturally-occurring non-hydrocarbon gases. Due to the possibility of different sources of the non-hydrocarbon gases across the region, multiple springs and rivers were sampled across a wide area of the state of Utah. Water samples were collected from non-stagnant regions of the sampled spring or river, and filtered through sterile 0.22 um Sterivex filter units. The biomass was extracted, amplified, and sequenced on the Illumina MiSeq platform.