Chemical and biological results, quality assurance and quality control, and method information from groundwater, surface water, and litter samples, collected from nine locations in Iowa and one in Wisconsin in 2016. Thirteen groundwater, nine surface water, four poultry litter, and four QA/QC samples were collected. Samples were analyzed at U.S. Geological Survey laboratories; bacteria, pathogens, and antibiotic resistance genes at the Michigan Bacteriological Research Laboratory, F+ specific coliphage at the St. Petersburg Coastal and Marine Science Center, hormones and antibiotics at the Organic Geochemistry Research Laboratory, nutrients at the National Water Quality Laboratory, total estrogenicity at Fish Health Laboratory.