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    • Mesophotic and Deep Benthic Communities (MDBC) eDNA metabarcoding: PC2202, NOAA Ship Pisces, June 30-July 11, 2022

    Metadata Updated: September 17, 2025

    Data from environmental DNA (eDNA) samples filtered from seawater collected in the Gulf of America during a research expedition on the NOAA Ship Pisces from June 30-July 11, 2022. Samples were collected at depths throughout the water column using Niskins mounted to rosettes on Connectivity-Temperature-Depth instruments (CTDs). Each eDNA sample was amplified using four primer sets: MiFish 12S rRNA marker targeting ray-finned fishes; 28S rRNA marker targeting corals and other cnidarians; and 18S rRNA and CO1 mtDNA general markers for eukaryotic plankton, protists, and other invertebrates. Sequences were then processed bioinformatically to infer amplicon sequence variants (ASVs) and assign taxonomic identifications from the WoRMs database. This data was collected as part of the Mesophotic and Deep Benthic Communities (MDBC) restoration project launched after the Deepwater Horizon oil spill. The data is formatted as per guidelines for upload to the Ocean Biodiversity Information System (OBIS, https://obis.org/).

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

    Downloads & Resources

    Dates

    Metadata Created Date September 12, 2025
    Metadata Updated Date September 17, 2025

    Metadata Source

    Harvested from DOI USGS DCAT-US

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date September 12, 2025
    Metadata Updated Date September 17, 2025
    Publisher U.S. Geological Survey
    Maintainer
    Identifier http://datainventory.doi.gov/id/dataset/usgs-682f1f29d4be024477811989
    Data Last Modified 2025-06-13T00:00:00Z
    Category geospatial
    Public Access Level public
    Bureau Code 010:12
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 9b4d5b05-f76c-49d6-8b41-225f39df3cdb
    Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
    Harvest Source Title DOI USGS DCAT-US
    Metadata Type geospatial
    Old Spatial -87.7710, 28.4437, -86.7318, 30.0812
    Source Datajson Identifier True
    Source Hash a55453e424fe4763cdc7ce04f2b1079a26d2fa6424d3c2ec23f8532488c74540
    Source Schema Version 1.1
    Spatial {"type": "Polygon", "coordinates": -87.7710, 28.4437, -87.7710, 30.0812, -86.7318, 30.0812, -86.7318, 28.4437, -87.7710, 28.4437}

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