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    • Measurement Quality Metrics to Improve Absolute Microbial Cell Counting

    Metadata Updated: March 14, 2025

    This repository contains the raw data and analysis scripts supporting the associated publication which introduces a framework to help researchers select fit-for-purpose microbial cell counting methods and optimize protocols for quantification of microbial total cells and viable cells. Escherichia coli cells were enumerated using four methods (colony forming unit assay, impedance flow cytometry - Multisizer 4, impedance flow cytometry - BactoBox, and fluorescent flow cytometry - CytoFLEX LX) and repeated on multiple dates. The experimental design for a single date starts with a cell stock that is divided into 18 sample replicates (3 each for 6 different dilution factors), and each sample is assayed one or two times for a total of 30 observations. Raw data files are provided from the Multisizer 4 (.#m4) and CytoFLEX LX (.fcs 3.0). The colony forming unit assay and BactoBox readings are recorded for each date as are the derived results from the Multisizer 4 and CytoFLEX LX. Also provided are an example analysis script for the *.fcs files and the statistical analysis that was performed.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    Dates

    Metadata Created Date March 14, 2025
    Metadata Updated Date March 14, 2025
    Data Update Frequency irregular

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date March 14, 2025
    Metadata Updated Date March 14, 2025
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-3410
    Data First Published 2025-02-13
    Language en
    Data Last Modified 2024-07-15 00:00:00
    Category Bioscience:Microbial measurements
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 8c7eca21-a537-4f20-ab14-8e5892877b9f
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-3410
    License https://www.nist.gov/open/license
    Program Code 006:045
    Source Datajson Identifier True
    Source Hash 6bffc075fa7be0b13f6e4261092c2cdf63d8a167cb69aaccd8dfd54f31c1a343
    Source Schema Version 1.1

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