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Marker residue depletion from the skin-on fillet tissue of rainbow trout exposed to AQUI S 20E: Data

Metadata Updated: July 6, 2024

Among the data required by the U.S. Food and Drug Administration (FDA) for approval of an aquaculture drug are the data that characterize the depletion of a drugs marker residue from the edible fillet tissue of fish after exposure. Eugenol is the marker residue for AQUI-S 20E, a product proposed for use as a sedative for fish.Rainbow trout (Onchorynchus mykiss; a representative cold water fish species) was exposed to AQUI-S 20E in water at a temperature of 9 C, a temperature that is representative of the lower range of temperatures where rainbow trout would be sedated. Eighty fish were exposed to a nominal AQUI-S 20E concentration of 100 mg/L for 60 min. Groups of 16 fish were sampled immediately after 60 min of exposure (the 0 min sample group), then at 15, 30, 90, and 150 min after the fish were transferred to fresh, flowing water. Skin-on fillets from each fish were analyzed for eugenol concentrations by using a FDA approved method for determining eugenol concentrations in fish fillet tissue. The method involved extracting eugenol from the tissue with acetonitrile, recovering eugenol from the tissue extract by using solid phase extraction techniques, and determining eugenol concentrations in the final extract by using high pressure liquid chromatography with absorbance detection techniques.Results indicated that maximum eugenol concentrations in the fillet tissue were measured immediately after the exposure (mean, 47.5 g/g). Eugenol concentrations decreased to 1.9 g/g by 150 min after the fish were transferred to fresh, flowing water. The depletion of eugenol from the fillet tissue was rapid (t1/2 = 31.7 min).

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Maintainer
@Id http://datainventory.doi.gov/id/dataset/ce28fd2a42c75804994887fa0bcf7a21
Identifier USGS:56659aafe4b06a3ea36c8691
Data Last Modified 20220616
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 02f1e1dd-b7a9-4a35-8ad1-0f65695d31e8
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash d6065b46257a244476625b6c28b76b91873fb97866b73603d4ef1dd544b9ec84
Source Schema Version 1.1

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