Acoustic sampling for presence of the endangered Hawaiian hoary bat (Lasiurus semotus, also known as Aeorestes semotus) was conducted at 17 stations across four Marine Corps Base Hawaii (MCBH) properties on O‘ahu Island, Hawai‘i. Bats were confirmed as present at all properties; MCBH Kāneʻohe Bay on Mōkapu Peninsula, Marine Corps Training Area Bellows (MCTAB) in Waimanalo, Camp H.M. Smith in Halawa Heights, and Pu'uloa Range Training Facility (RTF) on the 'Ewa coastal plain. Hawaiian hoary bats were recorded in airspace at all four properties during important periods of Hawaiian hoary bat life history, including periods of pregnancy, lactation, and pup fledging; however, overall presence was low. Foraging activity as identified from characteristic feeding buzzes was very rare and was recorded on only three nights over the entire study. Within-night bat detection pooled for all nights and stations at each property showed bat activity was mostly confined to the first several hours of the night at MCBH Kaneohe Bay and Pu'uloa RTF, whereas bat activity was spread throughout the night at Camp H.M. Smith and MCTAB. Recordings were analyzed with Kaleidoscope version 5.1.9h (Wildlife Acoustics Inc, Concord, Massachusetts). This data release consists of one tabular dataset including bat acoustic activity at all stations from February 2019 to March 2021.