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    • MAM Consortium Interlaboratory Study Raw Data

    Metadata Updated: October 15, 2022

    These LC-MS and LC-MS/MS raw data were collected for purposes of an interlaboratory study evaluating the multi-attribute method (MAM). Tryptic digests of native NISTmAb (the "Reference" and the "Unknown" samples), degraded NISTmAb (the "pH Stress" sample) and NISTmAb spiked with 15 heavy-labeled synthetic peptides (the "Spike" sample) were sent to each participating laboratory. One injection of each digest was acquired in MS-only mode, while a second injection was acquired in MS/MS mode. Three injections of a mixture of 15 heavy-labeled synthetic peptides ("Calibration" sample) were also analyzed as a means of evaluating instrument performance. Although all data were collected using the same C18 column and LC method, the instrumentation used by each laboratory differed. Additional details regarding the samples, their preparation, the LC method used, and an evaluation of the results pertaining to the new peak detection aspect of MAM can be found in "New Peak Detection Performance Metrics from the MAM Consortium Interlaboratory Study" (https://pubs.acs.org/doi/10.1021/jasms.0c00415). Evaluation of the results pertaining to the attribute analytics aspect of MAM can be found in "Interlaboratory Attribute Analytics Metrics from the MAM Consortium Round Robin Study" (link to be provided). Raw data that was optionally submitted by sixteen participating laboratories are provided here. To preserve the integrity of the data, the files are provided in their original vendor format.Please note that Linux and Mac users may require the use of 7Zip (https://www.7-zip.org/) to extract zipped folders, rather than the extraction tool provided by their operating system.For any difficulty with downloading or extracting data files, please e-mail the contact listed above.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    References

    https://doi.org/10.1021/jasms.0c00415
    https://doi.org/10.1021/jasms.2c00129

    Dates

    Metadata Created Date October 15, 2022
    Metadata Updated Date October 15, 2022
    Data Update Frequency irregular

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date October 15, 2022
    Metadata Updated Date October 15, 2022
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-2497
    Data First Published 2022-08-25
    Language en
    Data Last Modified 2021-10-18 00:00:00
    Category Bioscience:Chemistry
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 9b3953fb-0c16-49ae-b86b-184df7f74b44
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-2497
    License https://www.nist.gov/open/license
    Program Code 006:045
    Related Documents https://doi.org/10.1021/jasms.0c00415, https://doi.org/10.1021/jasms.2c00129
    Source Datajson Identifier True
    Source Hash 8e3b15f23554addb1d13e830dd0fa645f911eba7
    Source Schema Version 1.1

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