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Luminescence of AG129 mice infected with recombinant Monkeypox virus expressing firefly luciferase

Metadata Updated: July 6, 2024

Adult AG129 mice (129/Sv background deficient in alpha/beta interferon (IFN-α/β) and IFN-Ɣ receptors) were infected with recombinant Monkeypox virus (MPXV)expressing firefly luciferase by either intranasal (IN) or intraperitoneal (IP) routes. Experimental infections were conducted in a BSL-3 laboratory at the USGS National Wildlife Health Center, with a clade II MPXV that expresses firefly luciferase, as described in Osorio et. al, 2009 (MPXV/USA/luc). Twelve AG129 mice (8 males and 4 females) were infected intranasally with 105 plaque forming units (PFU) (5 ul, each nostril) of MPXV/USA/luc. Three control mice were inoculated in the same manner with an equal volume of PBS. Bioluminescent imaging was performed using an IVIS 200 series in vivo imager (Perkin Elmer, Oakland, CA). On days 19, 21, 23, and 25, post-infection, two to three mice were sacrificed to collect tissues. Twelve mice were also inoculated intraperitoneally with 105 PFU of the same virus diluted to 100 microliters of PBS. Three controls were injected intraperitoneally with the same volume of PBS. Two to three mice in this group were sacrificed on days 3, 5, 7, and 9 post-infection. Although tissues were collected, no further analysis was performed. Bioluminescent imaging was performed every other day from day 1 until day 29. Luminescence data was analyzed using Living Image software, version 3.2 (Perkin Elmer, Oakland, CA). Region of Interest (ROI) analysis was performed, using rectangular ROIs that covered the mouse body from nose to rump, excluding the tail unless significant luminescence was present on the tail. Luminescence within ROIs was quantified as total flux in photons per second (p/s).

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Identifier USGS:6419d6cdd34eb496d1d29c47
Data Last Modified 20230403
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id c572022a-f9d5-4543-b7f8-95b3adf731bb
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -89.485874,43.046374,-89.483278,43.050309
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash fed7d86aac1f33474d2792352151addb63ae1c1defe2a27ce31cec91ba814364
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -89.485874, 43.046374, -89.485874, 43.050309, -89.483278, 43.050309, -89.483278, 43.046374, -89.485874, 43.046374}

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