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    • LC Gradient Testing Study with RM 8321, E. coli digest, and HeLa digest

    Metadata Updated: December 15, 2023

    It may be possible to use a mixture of known peptides run on a linear gradient to create a model that can be used to predict an unknown LC gradient of unknown samples based on deviation from the model. Data from RM 8321 can also be used to test other de novo search algorithms since it is a known mixture of 439 peptides. This data set consists of LC-MS/MS analysis of RM 8321 on a linear gradient of five varied lengths (30 min, 60 min, 90 min, 120 min, and 240 min), which can be used for modeling or testing de novo searching. To facilitate future testing of LC gradient model performance, separate E. coli protein digest (Waters) and HeLa protein digest (Pierce) were separated on a typical two-step gradient. Specific LC gradient separations are described within and should be consulted. All separations, except ?130 min?, were run with the same data acquisition settings, and all were analyzed using a Thermo Fusion Lumos tribrid mass spectrometer running data-dependent acquisition using the orbitrap detector for both MS1 and MS2. These instrument method files are also available and named obviously.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    Dates

    Metadata Created Date December 15, 2023
    Metadata Updated Date December 15, 2023
    Data Update Frequency irregular

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date December 15, 2023
    Metadata Updated Date December 15, 2023
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-3028
    Data First Published 2023-10-02
    Language en
    Data Last Modified 2023-05-24 00:00:00
    Category Standards:Reference materials, Bioscience:Proteomics
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id bd1d8184-b066-4fec-a738-1c679d4be513
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-3028
    License https://www.nist.gov/open/license
    Program Code 006:045
    Source Datajson Identifier True
    Source Hash 12318e1ae691db6b1b61667758f9564bda377a2b50662bb4408d519d52b1b17d
    Source Schema Version 1.1

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