Assessing the distribution and abundance of both predator and prey (forage) fish species is a cornerstone of ecosystem-based fishery management, and supports decision making that considers food-web interactions. In support of binational Great Lakes fishery management the objectives of this survey were to: provide estimates of densities of key forage and predator species in the western basin of Lake Erie, to assess seasonal and spatial distributions of fishes in tandem with water quality information, and to assess year class strength. A systematic grid sampling approach with 41 stations was sampled via bottom trawl during June (Spring) and September (Autumn), starting in 2013. This data release adds 2021 data to the set for a total of nine years observation using the same gear and sampling design.