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Laboratory Incubation results from 2015 for bacterial cell counts, carbon use efficiency, growth efficiency, and dissolved organic matter chemistry from four glacier outflows and four rock glacier outflows in Colorado

Metadata Updated: July 6, 2024

Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The “Counts” tab is the number of cells counted in each view of the microscope using the acridine orange method. “C Calculations” tab is the calculation of carbon as bacterial biomass from cell counts. ”Change in cell counts” is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, “Carbon use efficiency” is calculated as the amount of DOM from oxygen demand assays converted to microbial biomass as carbon. The final two tabs “BGE with calculated RQ” and “BGE with RQ1” are the calculations of bacterial growth efficiency. The first uses a respiratory quotient calculated by using the change in CO2/the change in O2 from the biological oxygen demand assay measurements, and the second is calculated assuming a respiratory quotient of 1, a common practice in freshwater metabolism studies. Dissolved organic matter date is Metabolite Compounds as Identified through Gas Chromatography - Mass Spectrometry (GC-MS) analyses. The “GC-MS Data” tab is the intensities of each compound separated out by individual glacier and rock glacier. The second tab is a table of the top 25 compounds in each glacier and rock glacier, as identified through GC-MS, before and after the biological oxygen demand assays. Included in this table is the fold changes in the mass spectrometry intensities of individual compounds. The third tab is the statistical comparison of each glacier type before and after incubation. “Retention times”, the fourth tab, is the time of detection of each individual compound on the mass spectrometer. Finally, the last tab is a raw data file of all spectra generated through GC-MS including mass spec contaminates, which were removed prior to the metabolite analysis.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Maintainer
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Identifier USGS:5ca27c37e4b0b8a7f63172d1
Data Last Modified 20220714
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
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Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -105.7736,39.9308,-105.5374,40.3675
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash c5bbd0957879ec92defe0a2e10db932547fe3eb01d37e7e8f1813640e0069695
Source Schema Version 1.1
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