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Laboratory Incubation results from 2015 for bacterial cell counts, carbon use efficiency, growth efficiency, and dissolved organic matter chemistry from four glacier outflows and four rock glacier outflows in Colorado

Metadata Updated: November 19, 2025

Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The “Counts” tab is the number of cells counted in each view of the microscope using the acridine orange method. “C Calculations” tab is the calculation of carbon as bacterial biomass from cell counts. ”Change in cell counts” is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, “Carbon use efficiency” is calculated as the amount of DOM from oxygen demand assays converted to microbial biomass as carbon. The final two tabs “BGE with calculated RQ” and “BGE with RQ1” are the calculations of bacterial growth efficiency. The first uses a respiratory quotient calculated by using the change in CO2/the change in O2 from the biological oxygen demand assay measurements, and the second is calculated assuming a respiratory quotient of 1, a common practice in freshwater metabolism studies. Dissolved organic matter date is Metabolite Compounds as Identified through Gas Chromatography - Mass Spectrometry (GC-MS) analyses. The “GC-MS Data” tab is the intensities of each compound separated out by individual glacier and rock glacier. The second tab is a table of the top 25 compounds in each glacier and rock glacier, as identified through GC-MS, before and after the biological oxygen demand assays. Included in this table is the fold changes in the mass spectrometry intensities of individual compounds. The third tab is the statistical comparison of each glacier type before and after incubation. “Retention times”, the fourth tab, is the time of detection of each individual compound on the mass spectrometer. Finally, the last tab is a raw data file of all spectra generated through GC-MS including mass spec contaminates, which were removed prior to the metabolite analysis.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date September 13, 2025
Metadata Updated Date November 19, 2025

Metadata Source

Harvested from DOI USGS DCAT-US

Additional Metadata

Resource Type Dataset
Metadata Created Date September 13, 2025
Metadata Updated Date November 19, 2025
Publisher U.S. Geological Survey
Maintainer
Identifier http://datainventory.doi.gov/id/dataset/usgs-5ca27c37e4b0b8a7f63172d1
Data Last Modified 2022-07-14T00:00:00Z
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 9d0c94c1-acc3-4596-8888-0cab3c87799e
Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
Harvest Source Title DOI USGS DCAT-US
Metadata Type geospatial
Source Datajson Identifier True
Source Hash 5a05be4f85087a14d01eaf4a9f8edf0652f627926b716b20c2408a826f36f1c7
Source Schema Version 1.1

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