This dataset consists of select contaminants of emerging concern (CEC) including pesticides and transformation products, pharmaceuticals and transformation products, and wastewater indicator compound results measured in 131 surface water, 129 bottom sediment, 7 field replicate, and 6 field blank samples collected from 131 sites located on 27 tributaries of the Great Lakes during the summer of 2019. Samples were analyzed at the U.S. Geological Survey National Water Quality Laboratory (NWQL). Surface water samples were collected and analyzed for 238 pesticides, pesticide transformation products, and surrogate compounds (NWQL laboratory schedule 2437; Sandstrom and others, 2015), 152 pharmaceuticals, pharmaceutical transformation products, and surrogate compounds (NWQL laboratory schedule 2440; Furlong and others, 2014), and 65 wastewater indicator compounds and surrogate compounds (NWQL laboratory schedule 4433; Zaugg and others, 2007); bottom sediment samples were analyzed for 53 wastewater indicator compounds and surrogate compounds (NWQL laboratory schedule 5433; Burkhardt and others, 2007). In surface water, a total of 68 pesticides and transformation products, 20 pharmaceuticals, and 23 wastewater indicator compounds were detected in at least one sample. In bottom sediment, a total of 21 wastewater indicator compounds were detected in at least one sample.