The dataset contains micrographs of Hoplolaimus, Helicotylenchus, Meloidogyne, Mesocriconema, Pratylenchus, Trichodorus, and Tylenchorhynchus nematodes. The data were collected using 10×, 20× objectives with a Zeiss Observer Z1 fitted with a Zeiss Axiocam 503 color camera and with 10× and 40× objectives using an Olympus BX51 fitted with a DP74 Olympus camera. Data collected with the Zeiss microscope setup were processed with Zen blue version 2.6 and data collected with the Olympus microscope were processed with CellSens version 4.1. Individual images in the dataset were extracted from videos recorded with Zen blue and CellSens software.Micrographs are grouped by three objectives (10×, 20×, 40×) for seven nematode genera (Hoplolaimus, Helicotylenchus, Meloidogyne, Mesocriconema, Pratylenchus, Trichodorus, Tylenchorhynchus). Number of images:Helicotylenchus827Hoplolaimus995Mesocriconema527Meloidogyne433Pratylenchus666Trichodorus507Tylenchorhynchus1425