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    • Image data of growth of Valencia sweet orange nonembryogenic cells on points from a 3-factor response surface design to determine the effects of mineral nutrition on growth

    Metadata Updated: June 5, 2025

    The data are images of tissue cultures of Valencia sweet orange nonembryogenic callus cells taken in 2005 at the U.S. Horticultural Research Laboratory, Ft. Pierce, Florida, USA to photo document treatments from an experiment designed to determine the effects of mineral nutrition on callus growth using a 3-factor response surface methodology (RSM) design. The design matrix is presented in the spreadsheet file 3 factor RSM Design_7-1-2005.xlsx. The design included 20 runs. Six culture dishes were used to estimate the response for each run. A culture dish representative of each run was photographed. Each image is named to match the run number of the design. For example, the jpg image labeled Run 13_3F RSM_NE_Valencia_7-1-2005.JPG is an image of the callus grown on Run #13 of the 3-factor RSM design for the nonembryogenic Valencia sweet orange callus cells. This dataset includes 21 files – 20 image files and 1 Excel spreadsheet. Images were captured in JPEG (EXIF 2.2) format with a Nikon Coolpix 5400 digital camera equipped with a 1/1.8” (7.2 x 5.3 mm) CCD sensor at a resolution of 2592 x 1944 pixels. Each image is of a single culture plate with the top lid removed and photographed under cool white, fluorescent lighting.The experimental setup for the 5-factor response surface design is described in - Niedz, R. P. and T. J. Evens (2007). "Regulating plant tissue growth by mineral nutrition." In Vitro Cellular & Developmental Biology - Plant 43(4): 370-381.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: us-pd

    Downloads & Resources

    Dates

    Metadata Created Date November 2, 2024
    Metadata Updated Date June 5, 2025
    Data Update Frequency irregular

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date November 2, 2024
    Metadata Updated Date June 5, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/26663131.v1
    Data Last Modified 2025-05-02
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id c46fb659-a0cc-442c-96cb-6c1101d2ed34
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://www.usa.gov/publicdomain/label/1.0/
    Old Spatial {"type": "Point", "coordinates": -80.40894921548845, 27.427967681168788}
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash 26121a51e1073195fc8bc274ebdbac5a33a0becd30d8900a03cc68b84482392a
    Source Schema Version 1.1
    Spatial {"type": "Point", "coordinates": -80.40894921548845, 27.427967681168788}
    Temporal 2005-08-29/2005-08-29

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