Illumina sequencing data for MEC study on high biofilm conductivity in a Geobacter-enriched biofilm
This study systematically assessed intracellular electron transfer (IET) and extracellular electron transfer (EET) kinetics with respect to anode potential (Eanode) in a mixed-culture biofilm anode enriched with Geobacter spp. High biofilm conductivity (0.96–1.24 mScm^-1) was maintained during Eanode changes from -0.2 to +0.2 V versus the standard hydrogen electrode (SHE), although the steady-state current density significantly decreased from 2.05 to 0.35 Am^-2 in a microbial electrochemical cell. Substantial increase of the Treponema population was observed in the biofilm anode at Eanode=+0.2 V, which reduced intracellular electron-transfer kinetics associated with the maximum specific substrate-utilization rate by a factor of ten. This result suggests that fast EET kinetics can be maintained under dynamic Eanode conditions in a highly conductive biofilm anode as a result of shift of main EET players in the biofilm anode, although Eanode changes can influence IET kinetics.
This dataset is associated with the following publication:
Dhar, B., H. Ryu, H. Ren, J. Santodomingo, J. Chae, and H. Lee. High Biofilm Conductivity Maintained Despite Anode Potential Changes in a Geobacter-Enriched Biofilm. ChemSusChem. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, GERMANY, 9(24): 3485 –3491, (2016).
Resources
5 resources available
-
MEC-high biofilm conductivity-cDNA1_raw seqs.TXT
TEXT/PLAIN -
MEC-high biofilm conductivity-cDNA1_taxonomy.summary.xls
APPLICATION/VND.MS-EXCEL -
MEC-high biofilm conductivity-cDNA4_raw seqs.TXT
TEXT/PLAIN -
MEC-high biofilm conductivity-cDNA4_taxonomy.summary.xls
APPLICATION/VND.MS-EXCEL -
MEC-high biofilm conductivity_Illumina results_tables.docx
APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.WORDPROCESSINGML.DOCUMENT
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "020:00" ] |
| contactPoint |
{ "fn": "Hodon Ryu", "hasEmail": "mailto:ryu.hodon@epa.gov" } |
| description | This study systematically assessed intracellular electron transfer (IET) and extracellular electron transfer (EET) kinetics with respect to anode potential (Eanode) in a mixed-culture biofilm anode enriched with Geobacter spp. High biofilm conductivity (0.96–1.24 mScm^-1) was maintained during Eanode changes from -0.2 to +0.2 V versus the standard hydrogen electrode (SHE), although the steady-state current density significantly decreased from 2.05 to 0.35 Am^-2 in a microbial electrochemical cell. Substantial increase of the Treponema population was observed in the biofilm anode at Eanode=+0.2 V, which reduced intracellular electron-transfer kinetics associated with the maximum specific substrate-utilization rate by a factor of ten. This result suggests that fast EET kinetics can be maintained under dynamic Eanode conditions in a highly conductive biofilm anode as a result of shift of main EET players in the biofilm anode, although Eanode changes can influence IET kinetics. This dataset is associated with the following publication: Dhar, B., H. Ryu, H. Ren, J. Santodomingo, J. Chae, and H. Lee. High Biofilm Conductivity Maintained Despite Anode Potential Changes in a Geobacter-Enriched Biofilm. ChemSusChem. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, GERMANY, 9(24): 3485 –3491, (2016). |
| distribution |
[ { "title": "MEC-high biofilm conductivity-cDNA1_raw seqs.TXT", "mediaType": "text/plain", "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1379488/MEC-high%20biofilm%20conductivity-cDNA1_raw%20seqs.TXT" }, { "title": "MEC-high biofilm conductivity-cDNA1_taxonomy.summary.xls", "mediaType": "application/vnd.ms-excel", "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1379488/MEC-high%20biofilm%20conductivity-cDNA1_taxonomy.summary.xls" }, { "title": "MEC-high biofilm conductivity-cDNA4_raw seqs.TXT", "mediaType": "text/plain", "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1379488/MEC-high%20biofilm%20conductivity-cDNA4_raw%20seqs.TXT" }, { "title": "MEC-high biofilm conductivity-cDNA4_taxonomy.summary.xls", "mediaType": "application/vnd.ms-excel", "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1379488/MEC-high%20biofilm%20conductivity-cDNA4_taxonomy.summary.xls" }, { "title": "MEC-high biofilm conductivity_Illumina results_tables.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1379488/MEC-high%20biofilm%20conductivity_Illumina%20results_tables.docx" } ] |
| identifier | https://doi.org/10.23719/1379488 |
| keyword |
[ "Anode potential", "Microbial electrochemical cell", "biofilm conductivity", "electron transfer" ] |
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2017-09-06 |
| programCode |
[ "020:096" ] |
| publisher |
{ "name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": { "name": "U.S. Environmental Protection Agency", "subOrganizationOf": { "name": "U.S. Government" } } } |
| references |
[ "https://doi.org/10.1002/cssc.201601007" ] |
| rights |
null
|
| title | Illumina sequencing data for MEC study on high biofilm conductivity in a Geobacter-enriched biofilm |
