Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents

Metadata Updated: February 13, 2019

Cryptosporidiosis, giardiasis, and microsporidiosis are important waterborne diseases. In the standard for wastewater treatment plant (WWTP) effluents in China and other countries, fecal coliform is the only microbial indicator, raising concerns about the potential for pathogen transmission through WWPT effluent reuse. In this study, we collected 50 effluent samples (30 L/sample) from three municipal WWTPs in Shanghai, China and analyzed for Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi by microscopy and/or PCR. Moreover, propidium monoazide (PMA)-PCR was used to assess the viability of oocysts/cysts. The microscopy and PCR-positive rates for Cryptosporidium spp. were 62% and 40%, respectively. The occurrence rates of G. duodenalis were 96% by microscopy and 92–100% by PCR analysis of three genetic loci. Furthermore, E. bieneusi was detected in 70% (35/50) of samples by PCR. Altogether, ten Cryptosporidium species or genotypes, two G. duodenalis genotypes, and 11 E. bieneusi genotypes were found, most of which were human-pathogenic. The chlorine dioxide disinfection employed in WWTP1 and WWTP3 failed to inactivate the residual pathogens; 93% of the samples from WWTP1 and 83% from WWTP3 did not meet the national standard on fecal coliform levels. Thus, urban WWTP effluents often contain residual waterborne human pathogens.

This dataset is associated with the following publication: Ma, J., Y. Feng, Y. Hu, E. Villegas , and L. Xiao. Human infective potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in urban wastewater treatment plant effluents. JOURNAL OF WATER AND HEALTH. IWA Publishing, London, UK, 14(4): 411-423, (2016).

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

Downloads & Resources

References

http://jwh.iwaponline.com/content/early/2016/01/04/wh.2016.192

Dates

Metadata Created Date October 1, 2016
Metadata Updated Date February 13, 2019

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date October 1, 2016
Metadata Updated Date February 13, 2019
Publisher U.S. EPA Office of Research and Development (ORD)
Unique Identifier A-dfnd-295
Maintainer
Eric Villegas
Maintainer Email
Public Access Level public
Bureau Code 020:00
Schema Version https://project-open-data.cio.gov/v1.1/schema
Harvest Object Id e8e8dad9-98c7-4570-858b-d069fe2bf343
Harvest Source Id cf9b0004-f9fd-420e-bade-a86839e82acf
Harvest Source Title EPA ScienceHub
License https://pasteur.epa.gov/license/sciencehub-license.html
Data Last Modified 2016-09-21
Program Code 020:096
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents http://jwh.iwaponline.com/content/early/2016/01/04/wh.2016.192
Source Datajson Identifier True
Source Hash 721646e24942dd42fa6446c40ad74403c9d397a6
Source Schema Version 1.1

Didn't find what you're looking for? Suggest a dataset here.