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High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Catalog Last Checked: August 02, 2025 at 03:11 PM | Dataset Last Updated: March 17, 2023
This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.

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Complete Metadata
@type dcat:Dataset
accessLevel public
accrualPeriodicity irregular
bureauCode 
[
  "006:55"
]
contactPoint 
{
  "fn": "Anne L. Plant",
  "hasEmail": "mailto:anne.plant@nist.gov"
}
description This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.
distribution 
[
  {
    "title": "WSDOM iPSC Tracking GitHub Page",
    "format": "GitHub Python Files",
    "accessURL": "https://github.com/usnistgov/WSDOM",
    "description": "GitHub page with all scripts and models used for the image dataset."
  },
  {
    "title": "exp7",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2960/exp7.zip"
  },
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    "mediaType": "text/plain",
    "downloadURL": "https://data.nist.gov/od/ds/ark:/88434/mds2-2960/exp7.zip.sha256"
  },
  {
    "title": "Dataset_Information",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/Dataset_Information.xlsx"
  },
  {
    "title": "exp0",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp0.zip"
  },
  {
    "title": "exp1",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp1.zip"
  },
  {
    "title": "exp2-0",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-0.zip"
  },
  {
    "title": "exp2-1",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-1.zip"
  },
  {
    "title": "exp2-2",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-2.zip"
  },
  {
    "title": "exp2-3",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-3.zip"
  },
  {
    "title": "exp2-4",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-4.zip"
  },
  {
    "title": "exp2-5",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp2-5.zip"
  },
  {
    "title": "exp3-0",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-0.zip"
  },
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    "title": "exp3-1",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-1.zip"
  },
  {
    "title": "exp3-2",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-2.zip"
  },
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    "title": "exp3-3",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-3.zip"
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    "title": "exp3-4",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-4.zip"
  },
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    "title": "exp3-5",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp3-5.zip"
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    "title": "exp4-0",
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    "title": "exp4-2",
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    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp4-2.zip"
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    "title": "exp4-3",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp4-3.zip"
  },
  {
    "title": "exp4-4",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp4-4.zip"
  },
  {
    "title": "exp4-5",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp4-5.zip"
  },
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    "title": "exp5-0",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-0.zip"
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  {
    "title": "exp5-1",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-1.zip"
  },
  {
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    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-2.zip"
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    "title": "exp5-3",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-3.zip"
  },
  {
    "title": "exp5-4",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-4.zip"
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    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp5-5.zip"
  },
  {
    "title": "exp6-0",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp6-0.zip"
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  {
    "title": "exp6-1",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/exp6-1.zip"
  },
  {
    "title": "training_3DUNET",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/training_3DUNET.zip"
  },
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    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/training_high.zip"
  },
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    "title": "training_low",
    "mediaType": "application/zip",
    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/training_low.zip"
  },
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    "downloadURL": "https://data.nist.gov/od/ds/mds2-2960/training_medium.zip"
  }
]
identifier ark:/88434/mds2-2960
issued 2023-10-02
keyword 
[
  "AI",
  "U-Net",
  "genetic engineering; regenerative medicine",
  "pluripotent cell lines; quantitative live cell imaging; fluorescence microscopy; image data analysis; CNN"
]
landingPage https://data.nist.gov/od/id/mds2-2960
language 
[
  "en"
]
license https://www.nist.gov/open/license
modified 2023-03-17 00:00:00
programCode 
[
  "006:045"
]
publisher 
{
  "name": "National Institute of Standards and Technology",
  "@type": "org:Organization"
}
theme 
[
  "Bioscience:Cell biology"
]
title High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies

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