This release includes data and metadata (1-2) documenting visitation of ambrosia beetles (Coleoptera: Curculionidae) to healthy ʻōhiʻa (Metrosideros polymorpha) trees in two separate experiments, (3) geographical location and details of the trees used in the experiments, (4) culture of the fungal pathogens Ceratocystis lukuohia and Ceratocystis huliohia from naturally and experimentally infected ambrosia beetles, and (5) experimental transmission of the pathogens to healthy ʻōhiʻa seedlings by multiple species of ambrosia beetles. Data were used in the manuscript titled, "Ambrosia beetles (Coleoptera: Curculionidae) can directly transmit the fungal pathogens responsible for Rapid ʻŌhiʻa Death." This version of this data release contains an additional child item that was inadvertently omitted from the original release. First posted - December 13, 2022. Revised - April 4, 2023 (version 2.0 - contains all data from original release, plus one additional dataset)