HARV replication of the BRIC-21 Bacillus subtilis spaceflight samples
Previous spaceflight experiments using Bacillus subtilis have reported altered transcriptome profiles during spaceflight compared to matching ground samples. This study tried to replicate those transcriptome changes using High Aspect Ratio Vessels (HARVs). B. subtilis spores were grown in HARVs using the same protocol and growth conditions as the BRIC-21 spaceflight mission. RNA was extracted from the HARV samples and sequenced for differential expression analysis. HARV differentially expressed genes were then compared to the genes identified as differentially expressed in the BRIC-21 mission to access the accuracy of HARVs as a model for spaceflight experiments.
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Environmental and simulation facility conditions can modulate a behavioral-driven altered gravity response of Drosophila imagoes transcriptome
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | Previous spaceflight experiments using Bacillus subtilis have reported altered transcriptome profiles during spaceflight compared to matching ground samples. This study tried to replicate those transcriptome changes using High Aspect Ratio Vessels (HARVs). B. subtilis spores were grown in HARVs using the same protocol and growth conditions as the BRIC-21 spaceflight mission. RNA was extracted from the HARV samples and sequenced for differential expression analysis. HARV differentially expressed genes were then compared to the genes identified as differentially expressed in the BRIC-21 mission to access the accuracy of HARVs as a model for spaceflight experiments. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Environmental and simulation facility conditions can modulate a behavioral-driven altered gravity response of Drosophila imagoes transcriptome", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-34" } ] |
| identifier | nasa_genelab_GLDS-215_je2j-67g7 |
| issued | 2021-05-21 |
| keyword |
[ "library-construction", "microgravity-simulation", "nucleic-acid-extraction", "nucleic-acid-sequencing", "treatment-protocol" ] |
| landingPage | https://data.nasa.gov/dataset/harv-replication-of-the-bric-21-bacillus-subtilis-spaceflight-samples |
| license | http://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | HARV replication of the BRIC-21 Bacillus subtilis spaceflight samples |
