Invertebrate, fish, and habitat data were collected as part of a cooperative project that began in 2002 between the U.S. Geological Survey, Colorado Springs Utilities, and Colorado Springs Engineering. Other entities have contributed to the project through the years including Colorado Parks and Wildlife. Habitat samples were collected and analyzed at 27 sites – 25 in the Fountain Creek Basin as well as two sites on the Arkansas River, El Paso and Pueblo Counties, Colorado. The number of sites sampled each year as well as the number of annual visits during which data were collected and sampling methods used during each visit vary. The habitat data were collected between 2002 and 2024 and is intended to be updated annually as new data are collected and available. Users of these data are advised to read all metadata and associated documentation thoroughly to understand appropriate use and data limitations. NOTE: Although previous versions are available from the author, all the records in previous versions can be found in version 5.0. First posted - January 7, 2022 (available from author) Revised - May, 2022 (version 2.0) Revised - March, 2023 (version 3.0) Revised - March 2024 (version 4.0) Revised - May 2025 (version 5.0)