From July through December 2016, 56 environmental samples were collected from the Mohawk and Western New York River Basins. Samples were collected from nine production wells and 13 domestic wells in the Mohawk River Basin, and 17 production wells and 17 domestic wells in the Western New York River Basins. Samples were collected and processed using standard USGS methods and analyzed for 320 constituents including physicochemical properties, dissolved gases, major ions, nutrients, trace elements, pesticides, volatile organic compounds, radionuclides, and indicator bacteria. Analytical methods are described and referenced in Gaige and others (2023). Five of the Mohawk River Basin wells (HE 622, HE 624, OE1468, SA1501, and MT 406) were also sampled in 2002, 2006, and (or) 2011. Seven of the Western New York River Basins wells (AG 265, CU2131, E1903, E1904, GS 216, WO 351, and OL 19) were also sampled in 2006 and (or) 2011. Results from these wells and years are tabulated for comparison.