This U.S. Geological Survey (USGS) data release contains data from stream water, groundwater, and soil samples collected in 2019 and 2020 in the North Quartz Creek watershed in central Colorado. Fourteen streambank wells were installed in pairs at seven locations in August 2020 to capture the emerging groundwater from the left bank and right banks (relative to downstream-facing direction) and a synoptic sampling campaign was conducted to quantify metal contributions to the stream. A continuous, instream injection of sodium bromide (NaBr) was initiated at the head of the 5 km study reach several days prior to the synoptic sampling campaign and maintained throughout the duration of the study. Bromide concentrations were subsequently used to determine streamflow in the primary study reach (upper 1.3 km) using the tracer-dilution method, and as an indicator of hydrologic connections between North Quartz Creek and subsurface water. Streamflow was quantified in a secondary study reach (lower 3.7 km) using data from a series of sodium chloride slug additions wherein specific conductivity readings were used as a surrogate for the tracer concentration. Surface water samples were collected along North Quartz Creek including inflows from the left (LBI) and right (RBI) banks. Soil and sediment samples were collected along the transport path from source material (natural weathering and mine tailings/mine drainage) to the stream.