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Gas phase fractionation data independent acquisition analysis of a phosphopeptide mixture

Metadata Updated: May 9, 2023

Recent advances in methodology have made phosphopeptide analysis a tractable problem for many proteomicists. There are now a wide variety of robust and inexpensive enrichment strategies to generate phosphoproteomes, while free or inexpensive software tools for quantitation and site localization have simplified phosphoproteome analysis workflow tremendously. As a research group under the Association for Biomolecular Resource Facilities (ABRF) umbrella, the Proteomics Standards Research Group (sPRG) has worked to develop a multipathway phosphopeptide prototype mixture based on a pool of heavy-labeled phosphopeptides designed to enable researchers to rapidly develop assays. This prototype mixture contains 131 mass spectrometry vetted phosphopeptides specifically chosen to cover as many known biologically interesting phosphosites as possible from seven different signaling networks: AMPK signaling, death and apoptosis signaling, ErbB signaling, insulin/IGF-1 signaling, mTOR signaling, PI3K/AKT signaling, and stress (p38/SAPK/JNK) signaling. We describe a characterization of the standard spiked into a HeLa tryptic digest stimulated with both EGF and IGF1 to activate the MAPK and PI3K/AKT/mTOR pathways. We demonstrate a comparison of phosphoproteomic profiling of HeLa performed independently by the co-authors with this prototype mixture with data independent acquisition.

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Public: This dataset is intended for public access and use. License: See this page for license information.

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Metadata Created Date May 9, 2023
Metadata Updated Date May 9, 2023
Data Update Frequency irregular

Metadata Source

Harvested from NIST

Additional Metadata

Resource Type Dataset
Metadata Created Date May 9, 2023
Metadata Updated Date May 9, 2023
Publisher National Institute of Standards and Technology
Identifier ark:/88434/mds2-2843
Data First Published 2023-04-13
Language en
Data Last Modified 2022-11-09 00:00:00
Category Bioscience:Proteomics
Public Access Level public
Data Update Frequency irregular
Bureau Code 006:55
Metadata Context
Schema Version
Catalog Describedby
Harvest Object Id 88c6a081-ffdc-4b7c-a37e-17e07d649822
Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
Harvest Source Title NIST
Homepage URL
Program Code 006:045
Source Datajson Identifier True
Source Hash f0b3e3cc24ca504a82bae417719dee6b0042d961
Source Schema Version 1.1

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