When water is pumped slowly from saturated sediment-water inteface sediments, the more highly connected, mobile porosity domain is prefferentially sampled, compared to less-mobile pore spaces. Changes in fluid electrical conductivity (EC) during controlled downward ionic tracer injections into interface sediments can be assumed to represent mobile porosity dynamics, which are therefore distinguished from less-mobile porosity dynamics that is measured using bulk EC geoelectrical methods. Fluid EC samples were drawn at flow rates similar to tracer injection rates to prevent inducing preferential flow. The data were collected using a stainless steel tube with slits cut into the bottom (USGS MINIPOINT style) connected to an EC meter via c-flex or neoprene tubing, and drawn up through the system via a peristaltic pump. The data were compiled into an excel spreadsheet and time corrected to compare to bulk EC data that were collected simultaneously and contained in another section of this data release. Controlled, downward flow experiments were conducted in Dual-domain porosity apparatus (DDPA). Downward flow rates ranged from 1.2 to 1.4 m/d in DDPA1 and at 1 m/d, 3 m/d, 5 m/d, 0.9 m/d as described in the publication: Briggs, M.A., Day-Lewis, F.D., Dehkordy, F.M.P., Hampton, T., Zarnetske, J.P., Singha, K., Harvey, J.W. and Lane, J.W., 2018, Direct observations of hydrologic exchange occurring with less-mobile porosity and the development of anoxic microzones in sandy lakebed sediments, Water Resources Research, DOI:10.1029/2018WR022823.