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    • Experimental evaluation of nutrient and disease treatment effects on Acropora cervicornis (NCEI Accession 0299892)

    Metadata Updated: September 2, 2025

    Acropora cervicornis fragments of each genotype were evenly and haphazardly assigned to two nutrient treatments: ambient nutrients (Ambient) or elevated ammonium (NH4). Each nutrient treatment was replicated in four independent tanks (n = 3 fragments per genotype per tank). For ~1.5 months (47 d), Ambient tanks were maintained under nutrient levels consistent with the values in Virginia Key, FL, while elevated NH4 tanks were dosed with NH4Cl [3 mM] every 15 minutes using peristaltic pumps. The initial NH4 dose volume was 10 mL of the stock solution, targeting a ~10 μM increase in NH4 concentration. These values were calculated to account for the dilution of the nutrients resulting from adding new ambient seawater to the tanks (200 mL/min in a total tank volume of 180 L). After detecting higher than normal NH4 concentrations in the incoming seawater from Biscayne Bay, the NH4 dose volume was lowered to 5 mL of the stock solution, targeting ~5 μM NH4 increase above ambient values. The fragments were also assigned to disease vs. placebo treatments, the disease treatments involving exposure to homogenates of corals showing signs of white band disease following the protocol found in Rosales & Palacio-Castro (2024).

    Water samples (~40 mL) were collected to monitor NH4 levels in the treatments and immediately refrigerated at 4C. The elevated NH4 tanks were sampled daily, but the Ambient tanks were sampled less frequently (~2-3 days and no samples were collected during weeks 1 and 3 of the experiment). Nutrient concentrations were measured at NOAA-AOML using an AA3 nutrient analyzer (Seal Analytical, Southampton, UK). The instrument was calibrated before each run using standard solutions and procedures. Initially, only NH4 was monitored, but after high NH4 concentrations in the source seawater were detected, additional measurements of PO4 were included.

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    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date 2025-05-16T15:33:59Z
    Metadata Created Date February 1, 2025
    Metadata Updated Date September 2, 2025
    Reference Date(s) December 20, 2024 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NOAA/NESDIS/ncei/accessions

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    Additional Metadata

    Resource Type Nongeographicdataset
    Metadata Date 2025-05-16T15:33:59Z
    Metadata Created Date February 1, 2025
    Metadata Updated Date September 2, 2025
    Reference Date(s) December 20, 2024 (publication)
    Responsible Party (Point of Contact)
    Contact Email
    Guid gov.noaa.nodc:0299892
    Access Constraints Cite as: Palacio-Castro, Ana M.; Enochs, Ian C.; Kelble, Chris; Smith, Ian; Rosales, Stephanie M. (2024). Experimental evaluation of nutrient and disease treatment effects on Acropora cervicornis (NCEI Accession 0299892). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/s614-9c04. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
    Bbox East Long -80.1632
    Bbox North Lat 25.7324
    Bbox South Lat 25.7324
    Bbox West Long -80.1632
    Coupled Resource
    Frequency Of Update asNeeded
    Graphic Preview Description Preview graphic
    Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0299892
    Graphic Preview Type PNG
    Harvest Object Id 66f20b5e-374e-4666-a3a9-18166e35b2bd
    Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
    Harvest Source Title NOAA/NESDIS/ncei/accessions
    Licence accessLevel: Public
    Lineage
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Point", "coordinates": [-80.1632, 25.7324]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2020-08-12
    Temporal Extent End 2020-11-08

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