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    • Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data

    Metadata Updated: October 28, 2023

    This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between untreated iced samples and samples that received EtOH-NaAc treatment that were kept at room temperature. The data showed that DNA can be amplified from samples preserved using an EtOH-NaAc solution after at least seven days at room temperature.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date May 31, 2023
    Metadata Updated Date October 28, 2023

    Metadata Source

    Harvested from DOI EDI

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date May 31, 2023
    Metadata Updated Date October 28, 2023
    Publisher U.S. Geological Survey
    Maintainer
    @Id http://datainventory.doi.gov/id/dataset/cf90c97491930f45f2b6e702d1c3bbd9
    Identifier USGS:5aa28cebe4b0b1c392e8be5b
    Data Last Modified 20210909
    Category geospatial
    Public Access Level public
    Bureau Code 010:12
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://datainventory.doi.gov/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id c9852b02-2ea0-4ff4-bcd4-3c6fc86c88f3
    Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
    Harvest Source Title DOI EDI
    Metadata Type geospatial
    Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
    Source Datajson Identifier True
    Source Hash 5ca148bb26aa84cfd2409c9608a9d99c12f6b5745a90621a8915c9dd2cb03944
    Source Schema Version 1.1

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