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    • Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data

    Metadata Updated: October 29, 2025

    This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between untreated iced samples and samples that received EtOH-NaAc treatment that were kept at room temperature. The data showed that DNA can be amplified from samples preserved using an EtOH-NaAc solution after at least seven days at room temperature.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date September 12, 2025
    Metadata Updated Date October 29, 2025

    Metadata Source

    Harvested from DOI USGS DCAT-US

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date September 12, 2025
    Metadata Updated Date October 29, 2025
    Publisher U.S. Geological Survey
    Maintainer
    Identifier http://datainventory.doi.gov/id/dataset/usgs-5aa28cebe4b0b1c392e8be5b
    Data Last Modified 2021-09-09T00:00:00Z
    Category geospatial
    Public Access Level public
    Bureau Code 010:12
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id a4303d99-bb5c-4826-8ad0-5e4040f08cd0
    Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
    Harvest Source Title DOI USGS DCAT-US
    Metadata Type geospatial
    Source Datajson Identifier True
    Source Hash b817274894d3ca677e3958d650725195c2539bbb9f097adcbb7d1cb41c8b53f3
    Source Schema Version 1.1

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