Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution standards, are included when available for a method.