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    • EM122 Water Column Sonar Data Collected During AT26-10

    Metadata Updated: September 17, 2021

    AT26-10 Chemosynthetic Microbial Communities at Deep-Sea Vents (EM122). The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date September 14, 2021
    Metadata Created Date November 12, 2020
    Metadata Updated Date September 17, 2021
    Reference Date(s) January 15, 2014 (creation), February 1, 2015 (publication)
    Frequency Of Update notPlanned

    Metadata Source

    Harvested from NGDC MGG Sonar Water Column

    Other Data Resources

    Additional Metadata

    Resource Type Series
    Metadata Date September 14, 2021
    Metadata Created Date November 12, 2020
    Metadata Updated Date September 17, 2021
    Reference Date(s) January 15, 2014 (creation), February 1, 2015 (publication)
    Responsible Party NOAA National Centers for Environmental Information (Point of Contact)
    Contact Email
    Guid gov.noaa.ngdc.mgg.wcd:AT26-10_EM122
    Access Constraints Cite as: Rolling Deck to Repository (R2R). 2014. 'EM122 Water Column Sonar Data Collected During AT26-10'. NOAA National Centers for Environmental Information. [access date], Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose., Resource may not be available for up to one year after initial collection.
    Bbox East Long -102.3409446
    Bbox North Lat 12.98260485
    Bbox South Lat 9.76427495
    Bbox West Long -104.2929194
    Coupled Resource
    Frequency Of Update notPlanned
    Harvest Object Id 7c56f718-13c2-404d-a229-ceebeb178bfd
    Harvest Source Id ab8b0290-e4db-4a00-89f2-4e427c2054b2
    Harvest Source Title NGDC MGG Sonar Water Column
    Licence These data are considered raw and have not been subjected to the NOAA's quality control or quality assurance procedures. They are released for limited public use as preliminary data to be used only with appropriate caution. NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty., Not subject to copyright protection within the United States.
    Metadata Language eng; USA
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-104.2929194, 9.76427495], [-102.3409446, 9.76427495], [-102.3409446, 12.98260485], [-104.2929194, 12.98260485], [-104.2929194, 9.76427495]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System urn:ogc:def:crs:EPSG::4326
    Spatial Harvester True
    Temporal Extent Begin 2014-01-15T16:00:21
    Temporal Extent End 2014-01-20T03:35:18

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