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Disruption of Francisella noatunensis orientalis pdpA gene results in virulence attenuation and protection in zebrafish

Metadata Updated: July 6, 2024

Several Francisella spp. including F. noatunensis are regarded as important emerging pathogens of wild and farmed fish. However, very few studies have investigated the virulence factors that allow these bacterial species to be pathogenic in fish. The Francisella Pathogenicity Island (FPI) is a well-described, gene-dense region encoding major virulence factors for the genus Francisella. PdpA is a member of the pathogenicity determining protein genes encoded by the FPI that are implicated in the ability of the mammalian pathogen, F. tularensis, to escape and replicate in infected host cells. Using a sacB suicide approach, we generated pdpA knockouts to address the role of PdpA as a virulence factor for F. noatunensis. Since polarity can be an issue in gene-dense regions, we generated two different marker-based mutants in opposing polarity (FnoDpdpA1 and DpdpA2). Both mutants were attenuated (p<0.05), all of which could be restored to wild-type (WT) levels by complementation. Importantly, differences were found for bacterial burden and induction of acute phase and pro-inflammatory genes for Fno-pdpA1 and pdpA2 compared to WT during acute infection. In addition, neither mutant resulted in significant histopathological changes. Finally, immunization with Fno pdpA1 led to protection (p<0.012) against an acute lethal-dose 40 challenge with WT Fno in the zebrafish model of infection. The results from this study further demonstrate physiological similarities within the genus Francisella relative to their physiological relationships and the utility of zebrafish for addressing virulence factors for the genus.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Maintainer
@Id http://datainventory.doi.gov/id/dataset/b6fcbb0b9ed67ad44b53ad95930f8f2d
Identifier USGS:607f3f53d34e8564d680a2c0
Data Last Modified 20211028
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id b9a36d8b-9707-4d96-9f6f-e7b1d062a8da
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -122.2571683,47.6749963,-122.2563314,47.6752853
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 3a3f17078affcc1c376929f99c41e22dc321f4c596280b16099d2995f6d1e56d
Source Schema Version 1.1
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