To support research on dispersion in river channels, a tracer dye experiment was performed on the Kootenai River in northern Idaho, September 25-27, 2017. This data release provides access to digital aerial photography acquired during the experiment and the supporting field-based measurements of reflectance and dye concentration are available through related data releases. The digital aerial photography was acquired by the U.S. Fish and Wildlife Service using a Phase One iXU180-R mapping camera deployed from a Partenavia manned aircraft from a flying height of approximately 600 m above ground level. The data product included in this release is an orthorectified mosaic in JPEG2000 (*.jp2) format produced from a set of 45 images using Agisoft PhotoScan software. The resulting mosaic consists of 5.74 cm pixels with three bands: red, green, and blue (RGB). The mosaic encompasses the entrance to a meander bend that was the location of the plume of tracer dye at the time the images were acquired. The images were not radiometrically calibrated and consist of raw digital numbers. The UTC time period encompassed by the images comprising the mosaic was 16:37:27 to 16:41:29; this time information allows for synthesis with in situ measurements of Rhodamine WT dye concentration made during the experiment as the images were being acquired. Overall, the images and related field-based data sets were used to assess the potential for estimating tracer dye concentrations from remotely sensed data to support studies of dispersion in river channels.