Development and optimization of species-specific qPCR assays for eDNA detection of the mucket (Ortamannia ligamentina) and spectaclecase (Cumberlandia monodonta) mussels’ male mitotype
This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina and Cumberlandia monodonta from water samples (eDNA).
Find Related Datasets
Search by Tags
Click any tag below to search for similar datasets
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "Katy E Klymus", "@type": "vcard:Contact", "hasEmail": "mailto:kklymus@usgs.gov" } |
| description | This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina and Cumberlandia monodonta from water samples (eDNA). |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/P16VAVZF", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.66d1cb43d34ebebd6af05eb4.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_66d1cb43d34ebebd6af05eb4 |
| keyword |
[ "Boone County, Missouri", "Columbia Environmental Research Center", "USGS:66d1cb43d34ebebd6af05eb4", "biota", "environmental DNA", "mollusks", "polymerase chain reaction" ] |
| modified | 2024-09-04T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | -92.284999, 38.910784, -92.273644, 38.912612 |
| theme |
[ "geospatial" ] |
| title | Development and optimization of species-specific qPCR assays for eDNA detection of the mucket (Ortamannia ligamentina) and spectaclecase (Cumberlandia monodonta) mussels’ male mitotype |
