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De Facto Water Reuse: Bioassay suite approach delivers depth and breadth in endocrine active compound detection

Metadata Updated: March 15, 2021

Although endocrine disrupting compounds have been detected in wastewater and surface waters worldwide using a variety of in vitro effects-based screening tools, e.g. bioassays, few have examined potential attenuation of environmental contaminants by both natural (sorption, degradation, etc.) and anthropogenic (water treatment practices) processes. This study used several bioassays and quantitative chemical analyses to assess residence-time weighted samples at six sites along a river in the northeastern United States beginning upstream from a wastewater treatment plant outfall and proceeding downstream along the stream reach to a drinking water treatment plant. Known steroidal estrogens were quantified and changes in signaling pathway molecular initiating events (activation of estrogen, androgen, glucocorticoid, peroxisome proliferator-activated, pregnane X receptor, and aryl hydrocarbon receptor signaling networks) were identified in water extracts. In initial multi-endpoint assays geographic and receptor-specific endocrine activity patterns in transcription factor signatures and nuclear receptor activation were discovered. In subsequent single endpoint receptor-specific bioassays, estrogen (16 of 18 samples; 0.01 to 28 ng estradiol equivalents [E2Eqs]/L) glucocorticoid (3 of 18 samples; 1.8 to 21 ng dexamethasone equivalents [DexEqs]/L), and androgen (2 of 18 samples; 0.95 to 2.1 ng dihydrotestosterone equivalents [DHTEqs]/L) receptor transcriptional activation occurred above respective assay method detection limits (0.04 ng E2Eqs/L, 1.2 ng DexEqs/L, and 0.77 ng DHTEqs/L) in multiple sampling events. Estrogen activity, the most often detected, correlated well with measured concentrations of known steroidal estrogens (r2 = 0.890). Overall, activity indicative of multiple types of endocrine active compounds was highest in wastewater effluent samples, while activity downstream was progressively lower, and negligible in unfinished treated drinking water. Not only was estrogenic and glucocorticoid activity confirmed in the effluent by utilizing multiple methods concurrently, but other activated signaling networks that historically received less attention (i.e. peroxisome proliferator-activated receptor) were also detected.

This dataset is associated with the following publication: Medlock Kakaley, E., B. Blackwell, M. Cardon, J. Conley, N. Evans, D. Feifarek, E. Furlong, S. Glassmeyer, L. Gray, P. Hartig, D. Kolpin, M. Mills, L. Rosenblum, D. Villeneuve, and V. Wilson. De Facto Water Reuse: Bioassay suite approach delivers depth and breadth in endocrine active compound detection. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 699(134297): 1, (2020).

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Public: This dataset is intended for public access and use. License: See this page for license information.

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Metadata Created Date March 15, 2021
Metadata Updated Date March 15, 2021

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date March 15, 2021
Metadata Updated Date March 15, 2021
Publisher U.S. EPA Office of Research and Development (ORD)
Data Last Modified 2019-08-28
Public Access Level public
Bureau Code 020:00
Schema Version
Data Dictionary
Data Dictionary Type application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Harvest Object Id 75b41e6e-9edb-49a0-a56c-09e026fb9775
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
Program Code 020:096
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents
Source Datajson Identifier True
Source Hash fcf0bf73f2bec4114e2e4def4b26f483c2a0ac9c
Source Schema Version 1.1

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