There are 20 supplemental data files associated with this article. These files describe the process of assigning macroinvertebrate taxa to thermal preference categories and analyses associated with them. There are spreadsheets of macroinvertebrate taxa used in the publication and the resulting calculations for thermal preferences, including taxonomic relationships and variability associated with the taxonomic relationships (parent-child). Also included are the R scripts for calculating the Macroinvertebrate Thermal Tolerance Index (MTTI) and thermal preference metrics using the macroinvertebrate data. Additional macroinvertebrate data included in the files are: • Scatterplot of total number of individuals vs total number of taxa • Full list of BMI data sources • Map of BMI sampling sites, color-coded by data source • List of taxa that were in the tolerance analysis dataset but occurred in fewer than 30 samples For the plots showing probability of occurrence of a taxa, there is also a guide to assist with interpretation of the plots. Additional information for the MTTI includes: ● Calibration (CAL) and Validation (VAL) datasets ○ Summary Statistics ○ Boxplots of natural and disturbance variables ○ PCA biplots ● Modeling techniques ○ Deshrinking and tolerance downweighting ● Comparisons between field temperature and modeled temperatures (MWMT, MTTI) ● Correlations with other stressors Thermal metric plots for each MWMT class are also included.

This dataset is associated with the following publication: Hubler, S., J. Stamp, S.P. Sullivan, M. Fernandez, C. Larson, K. Macneale, R.W. Wisseman, R. Plotnikoff, and B. Bierwagen. Improved thermal preferences and a stressor index derived from modeled stream temperatures and regional taxonomic standards for freshwater macroinvertebrates of the Pacific Northwest, USA. ECOLOGICAL INDICATORS. Elsevier Science Ltd, New York, NY, USA, 160: 111869, (2024).