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Dataset for optical coherence tomography for label-free cell viability measurements in 3D tissue engineering scaffolds

Metadata Updated: April 18, 2023

In the field of tissue engineering, 3D scaffolds and cells are often combined to yield constructs that are used as therapeutics to repair or restore tissue function in patients. Viable cells are often required to achieve the intended mechanism of action for the therapy, where the live cells may build new tissue or may release factors that induce tissue regeneration. Thus, there is a need to reliably measure cell viability in 3D scaffolds as a quality attribute of a tissue-engineered medical product. Here, we developed a noninvasive, label-free, 3D optical coherence tomography (OCT) method to rapidly (2.5 min) image large sample volumes (1 mm) to assess cell viability and distribution within scaffolds. OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series OCT images demonstrated changes in the time-dependent speckle patterns due to refractive index (RI) variations within live cells that were not observed for pure hydrogel samples or hydrogels with dead cells. The changes in speckle patterns were used to generate live-cell contrast by image subtraction. In this way, objects with large changes in RI were binned as live cells. Using this approach, on average, OCT imaging measurements counted 326 ± 52 live cells per 0.288 mm for hydrogels that were seeded with 288 live cells (as determined by the acridine orange-propidium iodide cell counting method prior to seeding cells in gels). Considering the substantial uncertainties in fabricating the scaffold-cell constructs, such as the error from pipetting and counting cells, a 13% difference in the live-cell count is reasonable. Additionally, the 3D distribution of live cells was mapped within a hydrogel scaffold to assess the uniformity of their distribution across the volume. Our results demonstrate a real-time, noninvasive method to rapidly assess the spatial distribution of live cells within a 3D scaffold that could be useful for assessing tissue-engineered medical products.

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Public: This dataset is intended for public access and use. License: See this page for license information.

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Metadata Created Date April 18, 2023
Metadata Updated Date April 18, 2023
Data Update Frequency irregular

Metadata Source

Harvested from NIST

Additional Metadata

Resource Type Dataset
Metadata Created Date April 18, 2023
Metadata Updated Date April 18, 2023
Publisher National Institute of Standards and Technology
Identifier ark:/88434/mds2-2596
Data First Published 2023-03-21
Language en
Data Last Modified 2022-03-13 00:00:00
Category Health:Cell therapies
Public Access Level public
Data Update Frequency irregular
Bureau Code 006:55
Metadata Context
Schema Version
Catalog Describedby
Harvest Object Id f0206bb5-9452-47fe-8ff6-03b658fe6ed2
Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
Harvest Source Title NIST
Homepage URL
Program Code 006:045
Source Datajson Identifier True
Source Hash 5cd7cbda961b0193e618ae02cd1d06a9017aab04
Source Schema Version 1.1

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