Dataset for development and evaluation of a qPCR method modification that mitigates DNA recovery losses for analyses of E. coli fecal indicator bacteria in Great Lakes region recreational waters
Results previously reported by EPA/ORD on the modified method have led to its adoption into a Standard Operating Procedure for labs performing qPCR analyses for E. coli at recreational beaches in the state of Michigan. This dataset provides further documentation of the performance and applicability of the modified method for recreational beach water testing including data from an extensive survey that can be used to determine when and where unacceptable levels of DNA losses may occur with and without this method modification in the Great Lakes region.
Please see the attached dataset description document for additional information.
Resources
1 resource available
-
Haugland_QPCR Method Modification Dataset_Post External Review_final.xlsx
APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
Find Related Datasets
Search by Tags
Click any tag below to search for similar datasets
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Shawn Siefring",
"hasEmail": "mailto:siefring.shawn@epa.gov"
}
|
| description | Results previously reported by EPA/ORD on the modified method have led to its adoption into a Standard Operating Procedure for labs performing qPCR analyses for E. coli at recreational beaches in the state of Michigan. This dataset provides further documentation of the performance and applicability of the modified method for recreational beach water testing including data from an extensive survey that can be used to determine when and where unacceptable levels of DNA losses may occur with and without this method modification in the Great Lakes region. Please see the attached dataset description document for additional information. |
| distribution |
[
{
"title": "Haugland_QPCR Method Modification Dataset_Post External Review_final.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-dnd5/Haugland_QPCR%20Method%20Modification%20Dataset_Post%20External%20Review_final.xlsx"
}
]
|
| identifier | https://doi.org/10.23719/d-dnd5 |
| keyword |
[
"E. coli",
"Great Lakes Region",
"Interference",
"Michigan",
"QPCR methods comparisons"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2025-09-29 |
| programCode |
[
"020:000"
]
|
| publisher |
{
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
|
| references |
null
|
| rights |
null
|
| title | Dataset for development and evaluation of a qPCR method modification that mitigates DNA recovery losses for analyses of E. coli fecal indicator bacteria in Great Lakes region recreational waters |
