Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content
  • Feedback

    • Dataset for Absorbance Microscopy for Quantitative and Traceable Trypan Blue Cell Viability Measurement

    Metadata Updated: September 30, 2025

    The use of the microscope to assess cell viability is an inherently qualitative and subjective process. This project demonstrates a new absorbance microscopy modality for quantitative single-cell assessment of cell viability via trypan blue staining. This dataset consists of .tif images of live and dead stained and unstained Jurkat cells as well as the numerical data related to the corresponding journal article. The images were collected via brightfield microscopy through a 610nm bandpass filter. The data were collected to demonstrate a new imaging modality that enables the collection of traceable and comparable images of cells over time on the same microscope or on different microscopes. The processing of the brightfield images into absorbance images allows the intracellular uptake of trypan blue measured as moles/cell or mmol/L to be determined for individual cells. In this way, measurements of cell viability can be made in a quantitative, reproducible fashion. Quantitative measurements of cell viability are greatly needed to improve consistency in the biomanufacturing of cells and cell-based therapeutics. Note about Downloading: Please note that the pathlength for the download location must not exceed 260 characters; this is the pathlength limit in Windows. If you experience an error message about pathlength, then you can create a shorter pathlength for the download. For example, you could create a folder on C drive named 000 (triple zero) which would sort to the top of the list and would be C:\000 (6 characters). REFERENCE 1. Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021) Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy. Manuscript in preparation. 2. Babakhanova G, Zimmerman SM, Simon Jr CG (2021), Dataset for AbsorbanceQ App for Generating Absorbance Images from Brightfield Image Captures, National Institute of Standards and Technology, https://doi.org/10.18434/mds2-2423 (accessed June 22, 2021) 3. Zimmerman SM, Simon Jr CG, Babakhanova G (2021) AbsorbanceQ App for Generating Absorbance Images from Brightfield Image Captures. Manuscript in preparation.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    Dates

    Metadata Created Date September 30, 2025
    Metadata Updated Date September 30, 2025
    Data Update Frequency irregular

    Metadata Source

    Harvested from Commerce Non Spatial Data.json Harvest Source

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date September 30, 2025
    Metadata Updated Date September 30, 2025
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-2347
    Data First Published 2021-06-15
    Language en
    Data Last Modified 2021-01-07 00:00:00
    Category Bioscience, Bioscience:Cell biology, Bioscience:Biomaterials, Manufacturing:Biomanufacturing, Health:Cell therapies
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 996609ff-d75c-497e-955a-302362565c5d
    Harvest Source Id bce99b55-29c1-47be-b214-b8e71e9180b1
    Harvest Source Title Commerce Non Spatial Data.json Harvest Source
    Homepage URL https://data.nist.gov/od/id/mds2-2347
    License https://www.nist.gov/open/license
    Program Code 006:045
    Source Datajson Identifier True
    Source Hash f0b192db927d91f10d1a928ecbd5696bc655a88a8e94d4f86bfcc30b6c11c49a
    Source Schema Version 1.1

    Didn't find what you're looking for? Suggest a dataset here.