Actual evapotranspiration (ETa) rates from the Operational Simplified Surface Energy Balance (SSEBop) method, before and after bias corrections (Sepúlveda, 2021), are presented for basins located wholly or partially within the Southwest Florida Water Management District (SWFWMD). The SSEBop annual rates are provided at about a one square-kilometer scale from 2000 to 2017. Annual ETa rates calculated from the application of the water-balance method (wbETa) to 12 basins in the SWFWMD and annual land-use ETa (luETa) rates calculated from monthly average crop coefficient ratios are also provided in this data set. A GIS shapefile provides land-use type and annual ETa rate for each year of record. Data are tabulated in spreadsheets for each basin and are referred to by the name between parenthesis: Peace Creek Canal – Brush Lake basin (02293987), Saddle Creek at SR 542 near Lakeland basin (02294217), Joshua Creek at Nocatee-Honey Run basin (02297100), Prairie Creek at Fort Ogden basin (02298123), Shell Creek nr Punta Gorda basin (02298202), Myakka River near Sarasota basin (02298830), Big Slough at Tropicaire Blvd basin (02299450), Manatee River at Fort Hamer basin (02300021), Jumper Creek Canal near Bushnell basin (02312640), Panasoffkee Lake basin (02312700), Withlacoochee River near Holder basin (02313000), and Withlacoochee River Inglis & Bypass basin (02313230). Inflows and outflows from various sources in the water-balance equation used to calculate annual average wbETa rates for each basin in the SWFWMD area are presented in this dataset.