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    • Data Release for Replicate amphibian swab and standard dilution of artificial DNA data to assess qPCR and sampling detection error for Batrachochytrium dendrobatidis.

    Metadata Updated: July 27, 2024

    We generated a data set from by collecting duplicate swabs from all amphibian individuals sampled at 5 sites (96 individuals) and analyzed these duplicate swabs running 3 subsamples of the extracted DNA in separate qPCR reactions. The resulting data set included 2 swabs per individual with 1 qPCR result and a 2nd swab with 3 qPCR results for detection of Batrachochytrium dendrobatidis (Bd) DNA. We generated data to assess the ideal laboratory functional relationship between quantity of target Bd DNA and detection, as well as limits of detection (LOD) and quantification (LOQ) for the assay, 5 µL of synthetic double-stranded Bd DNA template (gBlocks™ Gene Fragments, Integrated DNA Technologies, Coralville, IA) ranging from 1.46 x 10-3 fg to 3 fg (approximately 6.6 – 13,000 copies) per reaction at 2-fold dilutions were each run 80 times on 10 separate plates (12 dilution series per plate).

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date July 27, 2024
    Metadata Updated Date July 27, 2024

    Metadata Source

    Harvested from DOI EDI

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date July 27, 2024
    Metadata Updated Date July 27, 2024
    Publisher U.S. Geological Survey
    Maintainer
    @Id http://datainventory.doi.gov/id/dataset/dd2e5fe94f57f7ff5b225ea2477afdab
    Identifier USGS:66a02911d34eef99d5abc7a9
    Data Last Modified 20240725
    Category geospatial
    Public Access Level public
    Bureau Code 010:12
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://datainventory.doi.gov/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 941a1d5f-18e1-412a-a891-9d0b07c2e38f
    Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
    Harvest Source Title DOI EDI
    Metadata Type geospatial
    Old Spatial -89.48581,43.048067,-89.483192,43.050294
    Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
    Source Datajson Identifier True
    Source Hash f3e29a72e325cb790e15102bd7d4920c91926956038e7a67d97a09d977bea59f
    Source Schema Version 1.1
    Spatial {"type": "Polygon", "coordinates": -89.48581, 43.048067, -89.48581, 43.050294, -89.483192, 43.050294, -89.483192, 43.048067, -89.48581, 43.048067}

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