Data were collected at the Flag Springs Complex on the Wayne E. Kirch Wildlife Management Area in Nevada to study the seasonal movements, habitat use, and survival of White River Spinedace. The dataset comprises information on PIT tagged White River Spinedace detected passively by remote antennas, along with habitat characteristics data. Length, weight, and species data were recorded during four bi-annual tagging events that occurred in June and November from November of 2020 to June of 2022. Passive detections of PIT tagged White River Spinedace were recorded at six locations throughout the flag springs complex. Meta data for each detection site are provided, including when antennas were operational. Water temperature data collected every hour at four sites in the study area are also included.