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Data on blood cells of the collector urchin, Tripneustes gratilla

Metadata Updated: October 29, 2023

Echinoderms such as urchins are important in marine ecosystems, particularly as grazers, and unhealthy urchins can have important ecological implications. For instance, unexplained mortalities of Diadema sp. in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately few tools exist to evaluate echinoderm health making management of mortalities or other health issues problematic. Hematology is often used to assess animal health in many animal groups including invertebrates but is seldom applied to echninoderms. We used a standard gravitometric technique to concentrate fixed ceolomocytes from the collector urchin Tripneustes gratilla onto microscope slides permitting staining and enumeration. Using Romanowsky stain and electron microscopy to visualize cell details, we found that in addition to amoebocytes, vibratile, clear and red spherule cells, Tripneustes has at least three other types of coelomocytes. Moreover, cytophagia of host cells by less than 1% of circulating amoebocytes is common (seen in 71% of sea urchins sampled). Cytophagocytic amoebocytes seems to target mainly the motile cells including red spherules, clear spherules, and vibratile cells disproportionate to underlying populations of these cell types. Lectins appear to bind to coelomocytes selectively and could be a useful biomarker for identifying or purifying echinoderm coelomocytes. The blood collection and smear preparation methods described here are simple, field portable, and might be a useful complementary tool for assessing health of other marine invertebrates and revealing heretofore unknown physiologic phenomena in this animal group.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023
Publisher U.S. Geological Survey
Maintainer
@Id http://datainventory.doi.gov/id/dataset/9e850328786f218355a4be5d04e73480
Identifier USGS:5ef4c5f982ced62aaae6732c
Data Last Modified 20201116
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id eedafb89-65d3-42b8-84b9-ba1ce75a0cff
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -158.5327,21.0179,-157.4396,21.938
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 5095081fa2b80b693ffc2281135cae96089cdf32f56e0f167987eb800e8bab43
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -158.5327, 21.0179, -158.5327, 21.938, -157.4396, 21.938, -157.4396, 21.0179, -158.5327, 21.0179}

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