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Data from: Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro

Published by Agricultural Research Service | Department of Agriculture | Catalog Last Checked: August 03, 2026 at 05:01 PM | Dataset Last Updated: June 11, 2026
To test the similarity of in vitro models of the gut microbiome to the originating microbiome, in this study fecal samples from four pigs were used as inoculum for in vitro incubations. At steady state, the incubations were sampled and compared to the fecal samples as well as to samples harvested directly from the corresponding region of the donor pig colon. Shotgun metagenomic sequencing and untargeted metabolomics data were generated. Untargeted metabolomics (median-normalized peak area data) and donor metadata are in this dataset with sample IDs linking to sequencing data housed in the NCBI Sequence Read Archive associated with BioProject PRJNA1163371.

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