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Data from: Photochemical and non-photochemical chlorophyll fluorescence dynamics in reproductive structures of semi-arid rangeland perennial bunchgrasses
These data were collected in a field study at the USDA-ARS Northern Great Basin Experimental Range, Oregon, USA, aimed at assessing the photochemical efficiency and ability of florets and flag leaves of two important sagebrush steppe bunchgrasses -- Crested wheatgrass (Agropyron cristatum), and Bluebunch wheatgrass (Pseudoroegneria spicata) -- to dissipate excess light energy over the 2025 plant reproductive period. Chlorophyll fluorescence measurements were made on flag leaves and distal and basal florets at night to determine dark-adapted baseline (Fo) and maximum fluorescence yields (Fm) and to calculate maximum photosystem II quantum efficiency (Fv/Fm = (Fm-Fo)/Fm). The same florets were then measured again the following morning when exposed to full incident ambient photosynthetic photon flux densities to determine light-adapted steady state fluorescence (Fs), maximum fluorescence yields (Fm') and light-adapted photosystem II quantum efficiency (PhiPSII = (Fm'-Fs)/Fs). Fs, Fm and Fm’ were used to estimate the quantum yield of non-photochemical energy loss (NPQ) in PS II that is physiologically regulated (PhiNPQ = (Fs/Fm’)-(Fs/Fm)) and unregulated (PhiNO = Fs/Fm). Data was collected five times over the entire reproductive cycle (pre-anthesis, anthesis, and post-anthesis), with concurrent average daily volumetric soil moisture at 10 cm soil depth (Vsm) from six Vsm observations made daily.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Hamerlynck, Erik P.", "hasEmail": "mailto:erik.hamerlynck@usda.gov" } |
| description | <p dir="ltr">These data were collected in a field study at the USDA-ARS Northern Great Basin Experimental Range, Oregon, USA, aimed at assessing the photochemical efficiency and ability of florets and flag leaves of two important sagebrush steppe bunchgrasses -- Crested wheatgrass (<a href="https://www.google.com/search?q=Agropyron+cristatum&ved=2ahUKEwimwYDi4baTAxWQ5skDHYH4EXUQgK4QegQIARAD" target="_blank"><i>Agropyron cristatum</i></a>), and Bluebunch wheatgrass (<a href="https://www.google.com/search?q=Pseudoroegneria+spicata&sca_esv=539c00fc8c0dc83a&ei=jZXBaerNBb7hp84PipK1cA&biw=1536&bih=825&ved=2ahUKEwj84NWL4raTAxX_4MkDHTvyIqEQgK4QegQIARAD&uact=5&oq=bluebunch+wheatgrass&gs_lp=Egxnd3Mtd2l6LXNlcnAiFGJsdWVidW5jaCB3aGVhdGdyYXNzMgUQLhiABDIKEAAYgAQYQxiKBTIFEAAYgAQyBRAAGIAEMgYQABgWGB4yBhAAGBYYHjIGEAAYFhgeMgYQABgWGB4yBhAAGBYYHjIGEAAYFhgeMhQQLhiABBiXBRjcBBjeBBjfBNgBAUipMVAAWABwAHgBkAEAmAFqoAFqqgEDMC4xuAEDyAEA-AEC-AEBmAIDoAKyMpgDALoGBggBEAEYFJIHCTAuMS44LTEuMaAHhw2yBwMwLjG4B3LCBwMyLTPIBxGACAA&sclient=gws-wiz-serp" target="_blank"><i>Pseudoroegneria spicata</i></a>) -- to dissipate excess light energy over the 2025 plant reproductive period. Chlorophyll fluorescence measurements were made on flag leaves and distal and basal florets at night to determine dark-adapted baseline (Fo) and maximum fluorescence yields (Fm) and to calculate maximum photosystem II quantum efficiency (Fv/Fm = (Fm-Fo)/Fm). The same florets were then measured again the following morning when exposed to full incident ambient photosynthetic photon flux densities to determine light-adapted steady state fluorescence (Fs), maximum fluorescence yields (Fm') and light-adapted photosystem II quantum efficiency (PhiPSII = (Fm'-Fs)/Fs). Fs, Fm and Fm’ were used to estimate the quantum yield of non-photochemical energy loss (NPQ) in PS II that is physiologically regulated (PhiNPQ = (Fs/Fm’)-(Fs/Fm)) and unregulated (PhiNO = Fs/Fm). Data was collected five times over the entire reproductive cycle (pre-anthesis, anthesis, and post-anthesis), with concurrent average daily volumetric soil moisture at 10 cm soil depth (Vsm) from six Vsm observations made daily.</p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "FPBData.xlsx", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "downloadURL": "https://ndownloader.figshare.com/files/61153936" } ] |
| identifier | 10.15482/USDA.ADC/31101193.v1 |
| keyword |
[ "Bluebunch wheatgrass", "Chlorophyll fluorescence", "Florets", "Fv/Fm", "PhiPSII", "Reproduction", "crested wheatgrass", "flag leaves", "non-photochemical quenching (NPQ)" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2026-03-30 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| spatial |
"{"type": "Point", "coordinates": [-119.691386, 43.472069000000005]}"
|
| temporal | 2025-06-13/2025-07-09 |
| title | Data from: Photochemical and non-photochemical chlorophyll fluorescence dynamics in reproductive structures of semi-arid rangeland perennial bunchgrasses |