Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content

Data from: Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP

Metadata Updated: July 11, 2025

This dataset accompanies the manuscript Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP | Scientific Reports (nature.com) published on 08/05/2024. The Orthoavulavirus javaense also called as Newcastle Disease virus is the causative of Newcastle disease a significant problem for the poultry industry. Virulent strains of this virus can cause extensive mortality and economic loss to the poultry industry. Current diagnostic methods rely on polymerase chain reaction targeting the fusion gene of this virus and this method is not only expensive and time consuming, but is also not field applicable. The dataset reported here and the manuscript published describe an assay that can be applied to field diagnosis of NDV via a colorimetric and fluorometric assay. The MB-RT-LAMP assay enables rapid detection of viruses from field samples without the need for RNA isolation and also enable rapid differentiation of virulent vs low virulence viruses. The data provided here demonstrate the sequence analysis undertaken to develop the MB-RT-LAMP primers and probes, the supporting data show the conditions tested to identify optimal parameters.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons Attribution

Downloads & Resources

Dates

Metadata Created Date November 2, 2024
Metadata Updated Date July 11, 2025
Data Update Frequency R/P1D

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 2, 2024
Metadata Updated Date July 11, 2025
Publisher Agricultural Research Service
Maintainer
Identifier 10.15482/USDA.ADC/26792542.v1
Data Last Modified 2025-06-03
Public Access Level public
Data Update Frequency R/P1D
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id d8b966a6-05f1-463b-9d27-5d1142db8dff
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://creativecommons.org/licenses/by/4.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash f2e9e2208da969cf1a568bc64560d7266c0f6f5478af2e8ca1d060f59db18425
Source Schema Version 1.1
Temporal 2023-03-01/2024-08-05

Didn't find what you're looking for? Suggest a dataset here.