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Data from: Chromosome-level genome assembly of Osmia taurus Smith (Hymenoptera: Megachilidae), an invasive bee species in North America

Published by Agricultural Research Service | Department of Agriculture | Catalog Last Checked: August 01, 2026 at 04:52 AM | Dataset Last Updated: March 16, 2026
Supplementary files for "Chromosome-level genome assembly of Osmia taurus Smith (Hymenoptera: Megachilidae), an invasive bee species in North America" Authors: Stephania Sandoval-Arango, Rena M. Schweizer, Michael G. Branstetter, Margarita M. Lopez-Uribe, Sheina B. Sim, Scott M. Geib, Jay D. Evans1The mason bee, Osmia taurus (Hymenoptera: Megachilidae), is native to Asia and was accidentally introduced to North America. This introduced species is now widespread and considered invasive due to negative impacts on native Osmia species. The number of introductions, invasion pathways, and mechanisms by which O. taurus has been able to establish and spread are currently unknown. Here, we present a high-quality genome assembly of O. taurus that will serve as a resource to study the genomic basis of its successful establishment in North America. We combined PacBio HiFi long-read sequencing, Hi-C short-read sequencing, and RNAseq data to assemble and annotate the genome. The genome of O. taurus is 536 Mb and represents one of the largest genomes sequenced in Megachilidae. About 70% of its genome is composed of repetitive elements. The supplementary data in this repository corresponds to annotation files generated during gene annotation with EGAPx, repeat annotation with RepeatModeler + RepeatMasker and Earlgrey, and the mitochondrial genome annotation. Additionally, we provide the complete phylogenetic tree generated using Ultraconserved elements extracted from the genomes of 16 megachilids, which was used to build Fig. 4. The analyses were performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas.These resources are meant to allow for future comparisons with additional related species and resquencing efforts for Osmia taurus, a bee species that is expanding its range across eastern North America.

Resources

10 resources available

  • Osmia_taurus-complete.proteins-EGAPx.faa

    FAA
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  • Osmia_taurus-complete.genomic-EGAPx.gff

    GFF
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  • Osmia_taurus-families-RepeatMasker.fa

    FA
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  • Osmia_taurus-families-Earlgrey.fa

    FA
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  • Osmia_taurus-filteredRepeats-Earlgrey.gff

    GFF
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  • Osmia_taurus-Repeats-RepeatMasker.gff

    GFF
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  • Osmia_taurus_cleanedFinal_mitogenome.gff

    GFF
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  • Osmia_taurus_cleanedFinal_mitogenome_mitos.fasta

    FASTA
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  • Read_me.txt

    TXT
    Download

  • Megachilidae-phylogeny-16t-f80-rooted.tre

    TRE
    Download

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Complete Metadata
@type dcat:Dataset
accessLevel public
accrualPeriodicity irregular
bureauCode 
[
  "005:18"
]
contactPoint 
{
  "fn": "Evans, Jay D.",
  "hasEmail": "mailto:jay.evans@usda.gov"
}
description <p dir="ltr">Supplementary files for "Chromosome-level genome assembly of <i>Osmia taurus</i> Smith (Hymenoptera: Megachilidae), an invasive bee species in North America" Authors: Stephania Sandoval-Arango, Rena M. Schweizer, Michael G. Branstetter, Margarita M. Lopez-Uribe, Sheina B. Sim, Scott M. Geib, Jay D. Evans1</p><p dir="ltr">The mason bee, <i>Osmia taurus</i> (Hymenoptera: Megachilidae), is native to Asia and was accidentally introduced to North America. This introduced species is now widespread and considered invasive due to negative impacts on native <i>Osmia </i>species. The number of introductions, invasion pathways, and mechanisms by which <i>O. taurus</i> has been able to establish and spread are currently unknown. Here, we present a high-quality genome assembly of <i>O. taurus</i> that will serve as a resource to study the genomic basis of its successful establishment in North America. We combined PacBio HiFi long-read sequencing, Hi-C short-read sequencing, and RNAseq data to assemble and annotate the genome. The genome of <i>O. taurus</i> is 536 Mb and represents one of the largest genomes sequenced in Megachilidae. About 70% of its genome is composed of repetitive elements. The supplementary data in this repository corresponds to annotation files generated during gene annotation with EGAPx, repeat annotation with RepeatModeler + RepeatMasker and Earlgrey, and the mitochondrial genome annotation. Additionally, we provide the complete phylogenetic tree generated using Ultraconserved elements extracted from the genomes of 16 megachilids, which was used to build Fig. 4. The analyses were performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas.</p><p dir="ltr">These resources are meant to allow for future comparisons with additional related species and resquencing efforts for <i>Osmia taurus</i>, a bee species that is expanding its range across eastern North America.</p><p><br></p>
distribution 
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    "@type": "dcat:Distribution",
    "title": "Osmia_taurus-complete.proteins-EGAPx.faa",
    "format": "faa",
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    "downloadURL": "https://ndownloader.figshare.com/files/61315567"
  },
  {
    "@type": "dcat:Distribution",
    "title": "Osmia_taurus-complete.genomic-EGAPx.gff",
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  {
    "@type": "dcat:Distribution",
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  {
    "@type": "dcat:Distribution",
    "title": "Osmia_taurus-families-Earlgrey.fa",
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    "@type": "dcat:Distribution",
    "title": "Osmia_taurus-Repeats-RepeatMasker.gff",
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  },
  {
    "@type": "dcat:Distribution",
    "title": "Osmia_taurus_cleanedFinal_mitogenome.gff",
    "format": "gff",
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    "@type": "dcat:Distribution",
    "title": "Osmia_taurus_cleanedFinal_mitogenome_mitos.fasta",
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  {
    "@type": "dcat:Distribution",
    "title": "Read_me.txt",
    "format": "txt",
    "mediaType": "text/plain",
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  {
    "@type": "dcat:Distribution",
    "title": "Megachilidae-phylogeny-16t-f80-rooted.tre",
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]
identifier 10.15482/USDA.ADC/31145080.v1
keyword 
[
  "bee",
  "beenome100",
  "genome",
  "i5K",
  "pollinator"
]
license https://creativecommons.org/publicdomain/zero/1.0/
modified 2026-03-16
programCode 
[
  "005:040"
]
publisher 
{
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}
temporal 2025-12-20/2025-12-20
title Data from: Chromosome-level genome assembly of Osmia taurus Smith (Hymenoptera: Megachilidae), an invasive bee species in North America

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