This dataset contains data in support of a study by the U.S. Geological Survey (USGS), in cooperation with the U.S. Army Corps of Engineers, to describe water-quality characteristics of the Red River of the North and tributaries prior to construction of the Flood Risk Management Project in the Fargo-Moorhead metropolitan area. This dataset contains bacteria data for ten sites supplied by the North Dakota Department of Environmental Quality, pesticide data for five sites supplied by the North Dakota Department of Agriculture, results from quality control samples collected at ten sites by the USGS as well as files necessary to run loads for ten sites and seven constituents published in U.S. Geological Scientific Investigations Report 2023-5136 [Galloway, J.G., Nustad, R.A., and Wheeling, S.L., 2023, Water-Quality Characteristics of the Red River of the North and Tributaries in the Fargo-Moorhead metropolitan area, North Dakota 2019-2022: U.S. Geological Survey Scientific Investigations Report 2023-5136]. For additional information see the metadata file specific to each dataset. The readme.txt file describes how to run the loads and water-quality and streamflow data used for computing loads is provided in this data release, but are available from the USGS National Water Information System.