This dataset contains all the raw data and R statistical software code needed to reproduce the maps and statistical analyses presented in the associated manuscript, titled “Virome of Solenopsis invicta (Hymenoptera: Formicidae): intercontinental differences in the native and introduced ranges.” In the manuscript, results are presented from a survey of viruses in 430 red imported fire ant nests at a total of 82 sites in its native range in Argentina and introduced range in the United States. A series of maps and graphs are generated to qualitatively describe the distribution of twenty different viruses, including prevalence of different social forms (monogyne and polygyne colonies) in each continent, and viral abundance by location and by season. In addition, we present a generalized linear mixed model that models the probability of virus presence as a function of viral species, continent, and social form. We investigate spatial patterning in model residuals and we compare the odds of each social form by virus within each continent and the odds of presence of each virus between the two continents. Raw data is provided in CSV form, and code is provided as an RMarkdown notebook along with its rendered HTML output. In addition, a GEOJSON file is included with the spatial coordinates of the study sites.FilesVirome survey S_invicta.csv: All raw data, including site-level covariates and coordinates of colony locations within each site. Column "GP9" indicates the social form of the sampled colony (Monogyne or Polygyne) and all following columns indicate presence (1) or absence (0) of 18 different virusesfire_ant_virome_code_supplement.Rmd: RMarkdown notebook with all code needed to import and process the data, generate maps and other figures, fit statistical models, and produce figures and tables with statistical model predictions.fire_ant_virome_code_supplement.html: Rendered output from code notebookfire_ant_sampling_sites.geojson: Spatial coordinates of the 82 sampling sites (unprojected latitude-longitude)